Mass Cytometry (CyTOF) Specialist
Company: Stanford University
Location: San Mateo
Posted on: January 27, 2023
Job Description:
The Human Immune Monitoring Center (HIMC) and Gates Center for
Human Systems Immunology is seeking a highly motivated Mass
Cytometry (CyTOF) Specialist (Life Science Research Professional 2)
to join our Cell Analysis group to perform multidisciplinary
projects related to CyTOF mass cytometry in human disease.
The mission of the HIMC is to provide standardized,
state-of-the-art immune monitoring assays at the RNA, protein, and
cellular level, as well as archiving, reporting, and data mining
support for clinical and translational studies. In partnership with
the research community, we also work to test and develop new
technologies for immune monitoring. The HIMC offers advanced CyTOF
mass cytometry panels for immunophenotyping, intracellular cytokine
staining, and phospho-signaling.
The Gates Center for Human Systems Immunology uses the
infrastructure of the HIMC to carry out mechanistic and correlative
science studies for clinical studies funded by the Bill and Melinda
Gates Foundation. These studies can include CyTOF assays, often
with significant study-specific customizations.
We are seeking a creative, motivated, organized individual to apply
and optimize CyTOF panels for the Gates Center and HIMC. The LSRP2
will collaborate with other team members and client investigators
to develop protocols, perform experiments, and evaluate and
interpret results.
Duties include:
- Independently conduct a portion of the research project(s).
Make recommendations on experimental design and/or research
direction to principal investigator.
- Independently conduct complex (lab and/or field) experiments to
gather data for a portion of a major research project.
- Interpret and analyze results, often where few guidelines
exist, and make modifications to existing procedures and
protocols.
- Develop new research protocols where appropriate protocols are
not described in the literature or where modification or adaptation
of standard procedures and protocols is required.
- Participate in multidisciplinary teams across different
faculties or schools.
- Perform ongoing literature review to remain current with new
procedures and related research; apply to ongoing research and
development of new protocols.
- Contribute substantively to the preparation of papers for
publication, especially in the results section, and to publication
of findings. Present ongoing work to colleagues and/or academic
conferences.
- Oversee supply and equipment budget under supervision of
principal investigator or business manager.
- Work with health and safety department to ensure lab complies
with regulations and all required trainings.
- Supervise (either formally or informally) staff or students as
needed, including oversight and direction on techniques, as well as
consultation on project work. Serve as a technical resource for
other research staff.
- Oversee the daily activities of the laboratory including lab
maintenance; maintain lab stock, manage chemical inventory and
safety records, and provide general lab support as needed. May
assist with purchasing laboratory equipment. - Other duties may
also be assigned
DESIRED QUALIFICATIONS:
- Hands-on experience with flow cytometry, or preferably, CyTOF
mass cytometry
- Experience with data analysis for flow cytometry/CyTOF and
competence with Excel and other data handling software. EDUCATION &
EXPERIENCE (REQUIRED):
Bachelor's degree in related scientific field and two years of
relevant work experience; or Master's degree in a related
scientific field.
KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):
- Comprehensive understanding of scientific principles.
- General computer skills and ability to quickly learn and master
computer programs, databases, and scientific applications.
- Strong analytical skills and excellent judgment.
- Ability to maintain detailed records of experiments and
outcomes.
- Ability to work under deadlines with general guidance is
essential.
- Excellent organizational skills and demonstrated ability to
accurately complete detailed work. PHYSICAL REQUIREMENTS :
- Frequently stand, walk, twist, bend, stoop, squat, grasp
lightly, use fine manipulation, grasp forcefully, perform
desk-based computer tasks, use telephone, write by hand, lift,
carry, push and pull objects weighing over 40 pounds.
- Occasionally sit, kneel, crawl, reach and work above shoulders,
sort and file paperwork or parts.
- Rarely climb, scrub, sweep, mop, chop and mix or operate hand
and foot controls.
- Must have correctible vision to perform duties of the job.
- Ability to bend, squat, kneel, stand, reach above shoulder
level, and move on hard surfaces for up to eight hours.
- Ability to lift heavy objects weighing up to 50 pounds.
- Ability to work in a dusty, dirty, and odorous
environment.
- Position may require repetitive motion. - Consistent with its
obligations under the law, the University will provide reasonable
accommodation to any employee with a disability who requires
accommodation to perform the essential functions of his or her
job.
WORKING CONDITIONS:
- May require working in close proximity to blood borne
pathogens.
- May require work in an environment where animals are used for
teaching and research.
- Position may at times require the employee to work with or be
in areas where hazardous materials and/or infectious diseases are
present.
- Employee must perform tasks that require the use of personal
protective equipment, such as safety glasses and shoes, protective
clothing and gloves, and possibly a respirator.
- May require extended or unusual work hours based on research
requirements and business needs. WORK STANDARDS:
- Interpersonal Skills: Demonstrates the ability to work well
with Stanford colleagues and clients and with external
organizations.
- Promote Culture of Safety: Demonstrates commitment to personal
responsibility and value for safety; communicates safety concerns;
uses and promotes safe behaviors bases on training and lessons
learned.
- Subject to and expected to comply with all applicable
University policies and procedures, including but not limited to
the personnel policies and other policies found in the University's
Administrative Guide, . As an organization that receives federal
funding, Stanford University has a COVID-19 vaccination requirement
that will apply to all university employees, including those
working remotely in the United States and applicable
subcontractors. To learn more about COVID policies and guidelines
for Stanford University Staff, please visit
Keywords: Stanford University, San Mateo , Mass Cytometry (CyTOF) Specialist, Other , San Mateo, California
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